Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescence Probing)

Product Description

AmoyDx® Novel Coronavirus (2019-nCoV) Detection Kit is a rapid and sensitive real-time RT-PCR assay that combines reverse transcription and PCR amplification in a one-step procedure for the qualitative detection of coronavirus SARS-CoV-2 with viral RNA extracted from human respiratory samples. The kit simultaneously detects ORF1ab (open reading frame, ORF1ab) and conserved regions of N (nucleoprotein) of the new coronavirus, with an internal control system to monitor the entire PCR process.

Principles of the Procedure

The kit is based on real-time RT-PCR technology, it combines reverse transcription and PCR amplification in a single-step procedure. It is designed for specific amplification of the conserved regions ORF1ab (open reading frame, ORF1ab) and N (nucleoprotein, N) of the new coronavirus SARS-CoV-2 in the viral RNA. The target region is amplified with various specific primers and detected with fluorescence probes. Internal non-competitive control is included in the nCoV RNA Detection System to assess RNA quality and monitor the entire PCR procedure.

The kit is composed of nCoV Reaction Mix, nCoV Enzyme Mix and nCoV Positive Control.

1) The nCoV reaction mix includes a nCoV RNA detection system and an internal control system. It contains primers and FAM-labeled probe specific for the ORF1ab gene of SARS-CoV-2, ROX-labeled probe specific for the N gene of SARS-CoV-2, and VIC-labeled probe specific for the internal control. The internal control system is designed for a housekeeping gene as a reference gene to assess the quality of the RNA and monitor the precision of the experimental operation, which may lead to false-negative results.

2) The nCoV enzyme mix contains reverse transcriptase for reverse transcription of viral RNA and reference gene RNA into cDNA, Taq DNA polymerase for PCR amplification, and uracil-N-glycosylase to prevent carryover contamination. PCR amplicon.

3) The nCoV positive control contains a nucleic acid template of the SARS-CoV-2 coronavirus ORF1ab and N genes and the housekeeping gene.

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