The VEGI/1283 Antibody conjugated with CF488A, a bright green fluorescent dye, is specifically designed for detecting Vascular Endothelial Growth Inhibitor (VEGI). VEGI, a member of the tumor necrosis factor (TNF) superfamily, plays a critical role in regulating vascular endothelial cell proliferation, angiogenesis, and apoptosis.
This antibody is particularly useful for fluorescence-based applications such as immunofluorescence (IF), flow cytometry (FC), and other imaging techniques, allowing for precise localization and quantification of VEGI expression in various biological samples.
Key Features
- Specificity: High specificity for VEGI (TNFSF15), ensuring minimal cross-reactivity with related proteins.
- Fluorophore Conjugation: Labeled with CF488A, a highly photostable green fluorescent dye with excitation/emission at 490/515 nm.
- High Sensitivity: Suitable for low-abundance target detection due to enhanced fluorescence intensity.
- Concentration: Provided at 0.1 mg/mL, ready for direct application in assays.
Applications
- Immunofluorescence (IF): For visualizing VEGI expression in tissue sections or cultured cells.
- Flow Cytometry (FC): For quantitative analysis of VEGI expression on cell surfaces or within cells.
- Confocal Microscopy: High-resolution imaging of VEGI distribution in biological systems.
- Angiogenesis Research: Evaluating VEGI’s role in endothelial cell function and vascular development.
Storage and Stability
- Storage Conditions: Store at 4°C in the dark. Avoid repeated freeze-thaw cycles to maintain antibody activity.
- Stability: Stable for at least 12 months from the date of purchase when stored properly.
Product Details
| Attribute | Specification |
|---|---|
| Clone | VEGI/1283 |
| Host | Rabbit |
| Conjugate | CF488A (Green) |
| Concentration | 0.1 mg/mL |
| Formulation | PBS, pH 7.4, with 0.09% sodium azide |
| Applications | IF, FC, Confocal Microscopy |
Recommended Protocols
1. Immunofluorescence
- Fix cells with 4% paraformaldehyde for 10 minutes.
- Permeabilize with 0.1% Triton X-100 for 5 minutes.
- Block with 5% BSA for 30 minutes.
- Incubate with VEGI/1283-CF488A antibody (dilution: 1:100) for 1 hour at room temperature.
- Wash with PBS and counterstain with DAPI for nuclear visualization.
- Mount and image using a fluorescence microscope.
2. Flow Cytometry
- Prepare a single-cell suspension and wash with PBS.
- Fix and permeabilize cells using an appropriate fixation buffer.
- Incubate cells with the VEGI/1283 antibody (dilution: 1:50) for 30 minutes on ice.
- Wash and analyze using a flow cytometer with a 488 nm laser.
Research Applications
- Cancer Research: VEGI’s role in tumor microenvironment regulation.
- Angiogenesis Studies: Exploring anti-angiogenic properties for therapeutic purposes.
- Vascular Diseases: Understanding VEGI’s function in pathological conditions like atherosclerosis or ischemia.
References
- Detailed studies and protocols are available from PubMed and NIH, highlighting VEGI’s role in vascular biology.
- Explore more about the CF488A dye and its applications at Biotium.
Disclaimer
This product is for research use only and not intended for diagnostic or therapeutic use. Ensure proper handling and disposal according to laboratory safety standards.
Let me know if you need further details or additional applications for this product!
