pENTR223.1 Plasmid

Abstract

pENTR223.1 plasmid -LC is a gateway cloning vector for bacterial expression. The vector is 3375 bp in size and contains the pBR322 origin of replication. This vector carries two genes, ccdB and CmR, in the region that will be replaced by cloned DNA segments. The ccdB gene (DNA gyrase inhibitor) provides strong negative selection against vector molecules that retain this region, while CmR (chloramphenicol resistance gene) provides positive selection for the propagation of the vector. The sequencing primers are T7, M13 and M13reverse. pENTR223.1-LC is resistant to spectinomycin up to 100 ug/ml.

Bottom

Valuable collections of clones encoding the complete ORFeomes for some model organisms have been built following the completion of their genome sequencing projects. These libraries are based on Gateway cloning technology, which facilitates the study of protein function by simplifying the subcloning of open reading frames (ORFs) into any suitable target vector.

The expression of proteins of interest as fusions with functional modules is a frequent approach in their initial functional characterization. A limited number of Gateway target expression vectors allow the construction of fusion proteins from ORFeome-derived sequences but are restricted to the possibilities offered by their built-in functional modules and predefined model organism specificity. Therefore, the availability of cloning systems that overcome these limitations would be highly advantageous.

Construction of pEF5/FRT/V5-DEST-R4-R3

Adaptation of pEF5/FRT/V5-DEST for MultiSite Gateway cloning was carried out as described by Magnani et al, with modifications. Briefly, an LR recombination reaction was carried out at 25 °C for 16 h between plasmids pEF5/FRT/V5-DEST_CamS (see above) and pDONR221-R4-R3. Importantly, the attR4/attR3 sites in pDONR221-R4-R3 cannot recombine with the juxtaposed attL1/attL2 sites. After transformation of the reaction into code Survival™ 2 T1R competent cells, cultures were plated on LB/agar plates containing ampicillin and chloramphenicol to select for colonies harbouring the recombined pEF5/FRT/V5-DEST plasmid.

In this way, the standard Gateway cassette flanked by attR1/attR2 in pEF5/FRT/V5-DEST was replaced by the Gateway MultiSite cassette flanked by attR4/attR3, obtaining pEF5/FRT/V5-DEST-R4-R3, which was ready for a MultiSite LR recombination cloning reaction with the three modules that would constitute the chosen fusion protein.

Molecular biology

The PCR reactions were carried out with AccuPrime Pfx SuperMix (Life Technologies) as indicated by the manufacturer. Plasmids pDONR and the cloning vector PDT-R4-R3 were obtained as part of the MultiSite Gateway Cloning Kit (Life Technologies Catalog No. 12537023). The pEF5/FRT/V5-DEST destination expression vector was obtained from Life Technologies (catalogue #V6020-20). This is a vector for cloning and expressing proteins in FLP-In™ isogenic cell lines.

The expression of cloned proteins is driven by the human elongation factor 1α promoter located upstream of the Gateway cassette. The vector has an FRT recombination site that mediates FLP recombinase-directed integration of the vector into a unique homologous FRT site in the genome of FLP-In™ cell lines, and the hygromycin resistance gene acts as a selectable marker for integration. However, it behaves like any other expression vector in transient transfection experiments.

BP and LR recombination reactions were carried out with BP clonase II and LR Clonase II Plus enzyme mixtures, respectively (Life Technologies), following the manufacturer’s instructions. Reactions were stopped by the addition of proteinase K and incubation at 37°C for 10 min. All competent strains of E. coli were obtained from Life Technologies and used in a one-shot format for plasmid transformation. LB medium was supplemented where indicated with selection antibiotics at the following concentrations: ampicillin (100 μg/mL), kanamycin (50 μg/mL), chloramphenicol (50 μg/mL).

Results

We present a versatile cloning toolkit for constructing fully customizable three-part fusion proteins based on the MultiSite Gateway Cloning System. The components of the fusion protein are encoded on the three integral plasmids of the kit. These can be recombined with any purposely designed target vector using a heterologous promoter external to the Gateway cassette, leading to in-frame cloning of an ORF of interest flanked by two functional modules.

Unlike previous systems, a third part is available for peptide encoding as it no longer needs to contain a promoter, resulting in a greater number of possible fusion combinations. We have constructed the component plasmids of the kit and demonstrated their functionality by providing proof-of-principle data on the expression of prototype fluorescent fusions in transiently transfected cells.

Conclusions

We have developed a set of tools to create fusion proteins with custom N- and C-term modules from Gateway input clones encoding ORFs of interest. Importantly, our method allows input clones obtained from ORFeome collections to be used without prior modification. Using this technology, any existing Gateway target expression vector with its template-specific properties could be easily adapted to express fusion proteins.

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pUC Plasmid

Abstract

A series of plasmid vectors containing the multiple cloning site (MCS7) of M13mp7 have been constructed. In one of these vectors, a kanamycin resistance marker has been inserted into the centre of the symmetrical MCS7 to produce a restriction site mobilizer (RSM) element. The drug resistance marker can be excised from this vector with any of the restriction enzymes that recognize a site in the RSM flanking sequences to generate an RSM with various sticky ends or blunt ends. These fragments can be used for insertional mutagenesis of any target molecule with compatible restriction sites.

Insertion mutants are selected for their resistance to kanamycin. When the drug resistance marker is removed with PstI, a small in-frame insert can be generated. In addition, two new MCS have been formed that have unique restriction sites by disrupting the symmetrical structure of MCS7. The resulting plasmids pUC8 and pUC9 allow separate double-digested restriction fragments to be cloned in both orientations relative to the lac promoter. The terminal sequences of any DNA cloned into these plasmids can be characterized using the M13 universal primers.

pUC plasmid vectors are used as cloning vectors and belong to the pUC series (named after the place of their initial preparation, ie, the University of California). These plasmids are 2700 bp long and contain

  • Ampicillin resistance gene
  • Origin of replication derived from pBR322,
  • lacZ gene derived from E. coli. Also found within the lac region is a polylinker sequence that has unique restriction sites (identical to those found in phage M13), and
  • Multiple Cloning Sites (MCS).

These plasmids, when transformed into an appropriate E.coli strain having lac (eg, JM103, JMI09), and grown in the presence of IPTG (isopropyl thiogalactoside, which behaves like lactose and induces lactose synthesis). of the enzyme galactosidase f3) and X-gal (substrate for the enzyme), will give rise to white or clear colonies.

On the other hand, the pUC that does not have inserts and is transformed into bacteria will have an active lacZ gene and will therefore produce blue colonies, which will make it possible to identify the colonies that have the pUC vector with cloned DNA segments.

As discussed above, in pBR322 and pBR327, the DNA is inserted into a site located in one of the two antibiotic resistance genes, thereby inactivating one of the two resistance genes. The insert-carrying plasmid can be selected for its ability to grow in a medium containing only one of the two antibiotics and for its inability to grow in a medium containing both antibiotics.

On the other hand, plasmids that do not carry an insert grow in media containing one or both antibiotics. Thus, the presence of the lacZ gene in pUC and ampicillin and tetracycline resistance genes in pBR322 and pBR327 allow selection of E. coli colonies transformed with plasmids carrying the desired foreign cloned DNA segment.

pUC19 is a commonly used high copy cloning vector. The vector encodes the N-terminal fragment of β-galactosidase (Lanza), which allows detection of blue/white colonies (i.e. complement a), as well as a pUC origin of replication and an ampicillin resistance gene that allow propagation and selection. in E. coli.

An important feature of pUC plasmids is the blue/white colony screen to detect recombinant plasmids. This selection is based on the inactivation of the Lanza peptide of beta-galactosidase, which is expressed by the vector. Cloning vectors belonging to the pUC family are available in pairs with reverse orders of restriction sites relative to the lacZ promoter. pUC8 and PUC9 form one such pair. Other similar pairs include pUC12 and pUCl3 or pUC18 and pUC19.

Disadvantages of pUC vectors

The basis of genomic sequence analysis is the large-scale cloning and sequencing of shotgun plasmid libraries obtained by assembling sequences from the vast majority of clones.

  • Breaches in shotgun libraries and unclonable DNA fragments are quite common. DNA is characterized by high AT content, strong secondary structure, open reading frames, or cis-acting functions (eg, transcriptional promoters or origins of replication).
  • In some cases, most notably in AT-rich DNA, the reasons for the difficulty in cloning are not well defined.
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Customized Plasmid

Abstract

Creative Biogene is a biotechnology company specializing in custom plasmid DNA production services. With years of experience servicing plasmid DNA production, Creative Biogene can offer custom plasmid services on microgram to gram scales using Creative Biogene’s unique and proprietary process. In addition, Creative Biogene can provide you with plasmids of various quality grades, including research-grade, endotoxin-free grade, and GMP grade plasmid DNA. Creative Biogene’s goal is to provide you with the most affordable high-quality plasmid DNA manufacturing services to ensure your satisfaction in a timely and professional manner.

Customized Plasmids are small DNA molecules that are physically separate from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular double-stranded DNA molecules. Today, plasmid DNAs serve as important tools in genetic engineering. They are commonly used in genetics and biotechnology laboratories, where they are used to clone and amplify (make many copies of) or express particular genes. Creative Biogene can provide you with high-quality plasmid DNA production services. You can select scales and grades of production quality to best meet your specific needs.

Creative Biogene can provide you with plasmids of multiple grades of quality. You can select the production-grade that best suits your needs.

  • Research Grade – For typical molecular biology research including recloning, sequencing, PCR, and protein manufacturing.
  • Endotoxin-free grade – recommended for effective cell transfection, genetic protein and vaccine manufacturing, and animal studies
  • GMP Grade: GMP grade plasmid DNA can be used for DNA vaccines, gene therapy, or ex vivo applications.

Creative Biogene offers customized plasmid DNA production services for your scientific research as follows:

  • Selection of the host strain and growth conditions.
  • High cell density fermentation.
  • Cell harvesting, lysis and purification of plasmids.
  • Wide range of quality control tests, including genomic RNA/DNA/protein contamination test, endotoxin (lipopolysaccharide) analysis, restriction profile analysis, DNA sequencing, OD260/280 readings, etc.
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t-Transglutaminase IgA ELISA

Product name

tissue transglutaminase IgA, tTG-IgA, ELISA kit

Full product name

Human Tissue Transglutaminase IgA, tTG-IgA ELISA kit

Product gene name

tTG-IgA ELISA kit

Species reactivity: Human

Specificity

No significant cross-reactivity or interference is observed between this analyte and analogues.

Samples

Human body fluids and tissue homogenates

Test type: Quantitative sandwich

Detection range: 31.2pg/ml-1000pg/ml

Sensitivity: 5.0pg/mL.

Intra-assay precision

Intra-assay CV (%) is less than 15%

Inter-assay precision

The inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not by OD values.

Preparation and Storage

Store all reagents at 2-8 degrees C

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.

Other notes

On occasion, small volumes of tTG-IgA ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Search terms for the purchase of tTG-IgA

MBS9302975 is a ready-to-use microwell and strip plate ELISA (Enzyme-Linked Immunosorbent Assay) Kit for testing the presence of Tissue Transglutaminase IgA, tTG-IgA ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing tTG-IgA.

The analytical biochemical ELISA technique of the MBS9302975 kit is based on tTG-IgA antibody-tTG-IgA antigen (immunosorbent) interactions and an HRP colourimetric detection system to detect tTG-IgA antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant tTG-IgA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).

Product related information for tTG-IgA ELISA kit

Background/Introduction: This quantitative sandwich ELISA kit is for laboratory reagents/research use only! This kit is intended to be used to determine the level of TSST-1 (hereinafter referred to as “analyte”) in undiluted original human body fluids and tissue homogenates. This kit is NOT suitable for testing non-biological sources of substances.

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe the intended use will not meet our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice.

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BioMaster HS-qPCR (2x)

Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance SYBR colourant-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of the DyNAmo HS SYBR Green qPCR Kit and added additional capabilities for your gene expression analysis.

The Thermo Scientific DyNAmo HS SYBR Green qPCR Kit contains a modified Thermus brockianus DNA polymerase and all other reagents required for qPCR. The user only needs to add PCR primers and template DNA. The modified DNA polymerase in this kit incorporates a nonspecific DNA-binding domain that provides physical stability to the DNA-polymerase complex. ROX Passive Reference Dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.

Reflexes

  • High specificity: DyNAmo HS SYBR Green Master Mix minimizes unwanted primer dimer formation and extension of non-specifically bound primers during reaction setup
  • Highly sensitive detection: Optimized master mix enables reproducible detection of low copy number templates
  • Wide Dynamic Range: The DyNAmo HS SYBR Green qPCR Kit detects as few as one copy of the target gene and achieves a dynamic range of up to eight orders of magnitude
  • Convenient to use – 2X master mix, just one tube. ROX passive reference dye provided in a separate vial

For research use only. It should not be used in diagnostic procedures.

Specifications

To use with (application): Real-time PCR (qPCR)

For use with (team)

System 7000, System 7300, Rapid System 7500, System 7500, System 7700, Rapid System 7900HT, System 7900HT, BioRad CFX96™, QuantStudio™, ViiA™ System 7, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Detection System for 5700 sequences, StepOnePlus™ System, Eppendorf MasterCycler RealPlex, Corbett RotorGene, StepOne™ System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Opticon, MJ Chromo4, Cepheid SmartCycler

Game type: DyNAmo HS SYBR Green qPCR Kit

Product line: Dynamo

Kind of product: SYBR real-time PCR master mix

Enough for: 500 reactions of 20 μL each, 200 reactions of 50 μL each

Concentration: 2X (DyNAmo HS SYBR Green qPCR Mix), 50X (ROX Dye)

Detection method: SYBR

Format: Tube

GC-Rich PCR Yield: High

No. of reactions: reactions

PCR method: qPCR

Polymerase: Tbr DNA polymerase

Reaction speed: Standard

Example type: DNA, dsDNA

Volume: 5x1ml

Content and storage

Contains:

  • 5 x 1 ml DyNAmo HS SYBR Green qPCR Mix (2X)
  • 250 µL of 50X ROX dye, 25 µM

Store at -20°C

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Detection Kit for 2019 Novel Coronavirus (2019-nCoV) RNA (PCR-Fluorescence Probing)

Product Description

AmoyDx® Novel Coronavirus (2019-nCoV) Detection Kit is a rapid and sensitive real-time RT-PCR assay that combines reverse transcription and PCR amplification in a one-step procedure for the qualitative detection of coronavirus SARS-CoV-2 with viral RNA extracted from human respiratory samples. The kit simultaneously detects ORF1ab (open reading frame, ORF1ab) and conserved regions of N (nucleoprotein) of the new coronavirus, with an internal control system to monitor the entire PCR process.

Principles of the Procedure

The kit is based on real-time RT-PCR technology, it combines reverse transcription and PCR amplification in a single-step procedure. It is designed for specific amplification of the conserved regions ORF1ab (open reading frame, ORF1ab) and N (nucleoprotein, N) of the new coronavirus SARS-CoV-2 in the viral RNA. The target region is amplified with various specific primers and detected with fluorescence probes. Internal non-competitive control is included in the nCoV RNA Detection System to assess RNA quality and monitor the entire PCR procedure.

The kit is composed of nCoV Reaction Mix, nCoV Enzyme Mix and nCoV Positive Control.

1) The nCoV reaction mix includes a nCoV RNA detection system and an internal control system. It contains primers and FAM-labeled probe specific for the ORF1ab gene of SARS-CoV-2, ROX-labeled probe specific for the N gene of SARS-CoV-2, and VIC-labeled probe specific for the internal control. The internal control system is designed for a housekeeping gene as a reference gene to assess the quality of the RNA and monitor the precision of the experimental operation, which may lead to false-negative results.

2) The nCoV enzyme mix contains reverse transcriptase for reverse transcription of viral RNA and reference gene RNA into cDNA, Taq DNA polymerase for PCR amplification, and uracil-N-glycosylase to prevent carryover contamination. PCR amplicon.

3) The nCoV positive control contains a nucleic acid template of the SARS-CoV-2 coronavirus ORF1ab and N genes and the housekeeping gene.

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100 mM dGTP

Description

Invitrogen dGTP is prepared at a final concentration of 100 mM in highly purified water. 100 mM dGTP is suitable for use in a polymerase chain reaction (PCR), sequencing, stuffing reactions, nick translation, cDNA synthesis, and TdT tracking reactions.

Invitrogen’s dNTP manufacturing meets the highest industry standards:

• Manufactured under ISO 9001
• Manufactured in Class D clean rooms

Features include:

  • Chemically synthesized
  • pH 7.5
  • >99% purity confirmed by HPLC
  • Stable for 2 years at –20°C
  • Free of qPCR, PCR, reverse transcription inhibitors
  • Free of DNases and RNases
  • Free of human and E. coli DNA

Specifications

Shipping condition: Approved for shipment on wet or dry ice

Concentration: 100mm

Label or tint: Unlabeled

Quantity: 250 litres

Content and storage

The nucleotide is supplied as a convenient, ready-to-use solution at a concentration of 100 mM in highly purified water (pH 7.5). Store at -20°C.

Applications:

  •  qPCR, RT-qPCR
  • PCR, RT-PCR, cDNA synthesis
  • High-fidelity, long-range PCR
  • Isothermal amplification
  • DNA marking
  • Cloning
  • Sanger sequencing and next-generation sequencing (NGS)

For research use only. It should not be used in diagnostic procedures.

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XEMA Test Peanut

Bottom

Peanut sensitization does not necessarily indicate a clinical peanut allergy, and uncertainty about whether or not a true peanut allergy exists can lead to increased anxiety and decreased quality of life for patients and their families. The gold standard for clinical diagnosis of peanut allergy is an oral food challenge, but this method is time-consuming and can cause severe allergic reactions. Therefore, it would be beneficial to develop a tool to predict clinical peanut allergy in peanut-sensitized individuals whose peanut allergy status is unknown to better determine who requires an oral food challenge for diagnosis.

Methods

Two separate studies were conducted. In Study 1, we recruited 100 participants from the McMaster University allergy clinic and community allergy outpatient clinics in the greater Hamilton area. We examined 18 different participant variables and used univariate and multivariate logistic regression analysis to determine how well these variables, alone and in combination, were able to predict clinical peanut allergy status.

In Study 2, we performed a retrospective chart review of a second cohort of 194 participants to investigate the reproducibility of our findings. This was a matched case-control study in which 97 peanut-allergic participants were matched for sex and age with 97 non-allergic control participants.

  • Skin prick test measurements

The forearm was prepped with alcohol and peanut extract (ALK-Pharmaceuticals, Mississauga, ON, Canada) was applied to the skin of the dorsal forearm. A sterile metal lancet (HollisterStier, Spokane, WA, USA) was used to pierce the skin below the allergen droplet. Wheal size was measured by skin prick test after 15 min. The tape was placed on the dorsal forearm and the outline of the wheel was traced. Two different study nurses measured the widest diameter of the wheel.

  • Plasma measurements of total and peanut IgE

Total IgE was measured with Image 800 (Beckman Coulter, Mississauga, ON, Canada) and peanut-specific IgE antibodies were measured with the Phadia 250 (Thermo Scientific, Waltham, MA, USA).

  • Cytokine measurements

Mononuclear cells were isolated from 30 to 40 ml of blood by density gradient centrifugation after lysing red blood cells with AKC lysis buffer. Cells were resuspended in RPMI supplemented with 10% FBS, 1% L-glutamine, 1% penicillin/streptomycin, 55 µM 2-mercaptoethanol (Thermo Scientific), 1 mM sodium pyruvate, 10 mM HEPES, and 0 MEM NEAA. 0.1 mM (Thermo Scientific). Scientific).

Viable cells were counted by Trypan Blue exclusion (Thermo Scientific) and resuspended at 8 × 10 6 cells/mL. 125,000 live cells per well were cultured in triplicate in medium alone or with 50 µg/mL/well crude peanut extract in flat-bottom 96-well plates (BD Biosciences, Mississauga, ON, Canada). After 5 days of culture at 37°C and 5% CO2, triplicates were pooled, centrifuged, and cell-free supernatants were collected and stored at -80°C until further analysis. Cytokines in cell-free supernatants were quantified using Luminex (Millipore Canada Ltd, Etobicoke, ON, Canada) following the manufacturer’s instructions.

  • Statistic analysis

Each predictor was entered into a univariate logistic regression analysis to determine if it was associated with the primary outcome: peanut allergy clinical status. We then generated cumulative models composed of multiple predictors using multivariable logistic regression. All univariate and multivariate analyzes included all 69 study participants from Groups 1, 2, and 4.

For all models, parameter estimates were obtained for each predictor and expressed as odds ratios with corresponding 95% confidence intervals and associated p-values. p-values ​​are reported to 4 decimal places.

Hierarchical models were compared to determine if the model with the greater number of predictors was statistically significantly better at predicting the primary outcome than the model with fewer predictors. This was done by comparing the −2 Log-Likelihood statistics of the models. For each model, the area under the receiver operating characteristic (ROC) curve was reported as a measure of discriminability.

The best model was used to predict the peanut allergy status of Group 3 participants and to determine the predicted probability (Pr) that each participant had a clinical peanut allergy. Using Pr, we classify each individual as peanut allergic or not, based on a specific cutoff point. We chose this cutoff point to eliminate false negatives and maximize true positives in the data set.

Results

Peanut skin prick test wheal size was the best predictor of clinical peanut allergy in both study cohorts. For every 1-mm increase in wheel size, the odds ratio that an individual had a clinical peanut allergy was 2.36 in our first cohort and 4.85 in our second cohort. No other variable came close to the predictive power of wheal size.

Conclusions

Peanut skin prick test wheel size is a strong predictor of clinical reactivity to peanuts. The findings of this study may be useful in guiding physicians’ decision-making regarding the diagnosis of peanut allergy.

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Vitamin D deficiency in critically ill COVID-19 ARDS patients

Vitamin D’s pleiotropic results embody immune modulation, and its supplementation has been proven to forestall respiratory tract infections. The effectivity of vitamin D as a therapeutic intervention in important sickness stays much less outlined. The present examine analyzed medical and immunologic results of vitamin D ranges in patients affected by coronavirus illness 2019 (COVID-19) induced acute respiratory misery syndrome (ARDS).
The overwhelming majority of COVID-19 ARDS patients had vitamin D deficiency. 25-hydroxyvitamin D standing was not associated to adjustments in medical course, whereas low ranges of 1,25-dihydroxyvitamin D have been related to extended mechanical air flow and a worse APACHE II rating. Clinical parameters didn’t differ between patients with adequate or poor ranges of 25-hydroxyvitamin D. Only circulating plasmablasts have been increased in patients with 25-hydroxyvitamin D ranges ≥30 ng/ml (p = 0.029).
 This was a single-center retrospective examine in patients receiving intensive care with a confirmed SARS-CoV-2 an infection and COVID-19 ARDS. 25-hydroxyvitamin D and 1,25-dihydroxyvitamin D serum ranges, pro- and anti inflammatory cytokines and immune cell subsets have been measured on admission in addition to after 10-15 days. Clinical parameters have been extracted from the affected person knowledge administration system. Standard working procedures included the every day administration of vitamin D3 through enteral feeding.
This was a quasi-experimental examine utilizing a nonequivalent management group pretest-posttest non-synchronized design and blended strategies. The pattern comprised of 56 patients, with 28 every in the experimental and management teams. The management group was recruited from May 25-June 19, 2018, and the experimental group from September 1-13, 2018.
Self-efficacy and self-care adherence have been assessed through questionnaires; consumer experiences have been assessed through interviews after the appliance was used for four weeks. The well being diary utility is anticipated to encourage coronary heart transplant recipients to carry out self-care and enhance adherence to medical recommendation.
A complete of 39 patients with COVID-19 ARDS have been eligible, of which 26 have been included in this examine as knowledge on vitamin D standing was out there. 96% suffered from extreme COVID-19 ARDS. All patients with out prior vitamin D supplementation (n = 22) had poor serum ranges of 25-hydroxyvitamin D. Vitamin D supplementation resulted in increased serum ranges of 25-hydroxyvitamin D however not didn’t improve 1,25-dihydroxyvitamin D ranges after 10-15 days.

Synergistic Action of Diclofenac with Endotoxin-Mediated Inflammation Exacerbates Intestinal Injury in Vitro

Intestinal homeostasis is tightly regulated by the orchestrated actions of a large number of cell sorts, together with enterocytes, goblet cells, and immune cells. Disruption of intestinal barrier perform can improve susceptibility to pathogen invasion and destabilize commensal microbial-epithelial-immune interplay, manifesting in varied intestinal and systemic pathologies. However, a quantitative understanding of how these cell sorts talk and collectively contribute to tissue perform in well being and illness is missing.

Here, we utilized a human intestinal epithelial-dendritic cell mannequin and multivariate evaluation of secreted components to research the mobile crosstalk in response to physiological and/or pathological cues (e.g., endotoxin, nonsteroidal anti-inflammation drug (NSAID)). Specifically, we demonstrated that remedy with diclofenac (DCF), an NSAID generally used to deal with irritation related to acute an infection and different circumstances, globally suppressed cytokine secretion when dosed in isolation.

However, the disruption of barrier perform induced by DCF allowed for luminal lipopolysaccharide (LPS) translocation and activation of resident immune cells that overrode the anti-inflammatory affect of DCF. DCF-facilitated irritation in the presence of LPS was in half mediated by upregulation of macrophage migration inhibitory issue (MIF), an necessary regulator of innate immunity. However, whereas neutralization of MIF exercise normalized irritation, it didn’t result in intestinal therapeutic.

Our knowledge counsel that methods-wide suppression of irritation alone is inadequate to realize mucosal therapeutic, particularly in the presence of DCF, the goal of which, the COX-prostaglandin pathway, is central to mucosal homeostasis. Indeed, DCF removing postinjury enabled partial restoration of intestinal epithelium capabilities, and this restoration section was related to upregulation of a subset of cytokines and chemokines, implicating their potential contribution to intestinal therapeutic.

The outcomes spotlight the utility of an intestinal mannequin capturing immune perform, coupled with multivariate evaluation, in understanding molecular mechanisms governing response to microbial components, supporting utility in learning host-pathogen interactions. Patients with 1,25-dihydroxyvitamin D ranges under 20 pg/ml required longer mechanical air flow (p = 0.045) and had a worse acute physiology and persistent well being analysis (APACHE) II rating (p = 0.048).

Vitamin D deficiency in critically ill COVID-19 ARDS patients

Inhaled vaccine supply in the fight towards respiratory viruses: a 2021 overview of latest developments and implications for COVID-19

As underlined by the late 2019 outbreak of extreme acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), vaccination stays the cornerstone of worldwide well being-care. Although vaccines for SARS-CoV-2 are being developed at a record-breaking tempo, the vast majority of these which can be licensed or at the moment registered in medical trials are formulated as an injectable product, requiring a tightly regulated cold-chain infrastructure, and primarily inducing systemic immune responses.

Here, we make clear the standing of inhaled vaccines towards viral pathogens, offering background to the position of the mucosal immune system and elucidating what components decide an inhalable vaccine’s efficacy. We additionally focus on whether or not the event of an inhalable powder vaccine formulation towards SARS-CoV-2 could possibly be possible. The evaluate was carried out utilizing related research from PubMed, Web of Science and Google Scholar

Bovine IgM, purified (non-immune, serum)

20001-2-1 0.5 mg
EUR 225

Bovine IgA (non-immune, serum), purified

20001-3-1 100 ug
EUR 529

Mouse (non-immune) Serum IgM, purified

20008-2 100 ug
EUR 141

Mouse (non-immune) Serum IgG, purified

20008-25 25 mg
EUR 469

Chicken (non-immune) Serum IgM, purified

20010-2-1 0.5 mg
EUR 225

Goat (non-immune) Serum IgM, purified

20011-2-1 0.5 mg
EUR 225

Dog (non-immune) Serum IgM, purified

20016-2-1 0.5 mg
EUR 225

Mouse serum (non-immune/control), C57BL6

NMOS-1CS 1 ml
EUR 141

Bovine Serum Control IgG (non-immune) purified

20001-1 1 mg
EUR 141

Bovine IgG1 (Serum Control, non-immune) purified

20001-G1 100 ug
EUR 141

Bovine IgG2 (Serum Control, non-immune) purified

20001-G2 100 ug
EUR 141

Monkey Serum IgM (Rhesus, non-immune), purified

20013-1 50 ug
EUR 164

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-1 1 mg
EUR 141

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder

20009-WA-5 5 mg
EUR 286

Rabbit Immune ribonucleic acid/immune RNA ELISA kit

E04I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Immune ribonucleic acid/immune RNA ELISA kit

E04I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Immune ribonucleic acid/immune RNA ELISA kit

E04I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Hamster (Syrian, non-immune) Serum IgM, purified (Syrian)

20003-2-1 0.5 mg
EUR 225

Human IgG, purified (serum, non-immune, isotype control)

20007-1-1 1 mg
EUR 141

Human IgG, purified (serum, non-immune, isotype control)

20007-1-100 100 mg
EUR 895

Human IgG, purified (serum, non-immune, isotype control)

20007-1-25 25 mg
EUR 651

Human IgG, purified (serum, non-immune, isotype control)

20007-1-5 5 mg
EUR 286

Mouse IgG, purified (serum non-immune, isotype control)

20008-1 1 mg
EUR 141

Mouse IgG, purified (serum non-immune, isotype control)

20008-100 100 mg
EUR 598

Mouse IgG, purified (serum non-immune, isotype control)

20008-5 5 mg
EUR 286

Goat IgG, purified (serum non-immune, isotype control)

20011-1 1 mg
EUR 141

Goat IgG, purified (serum non-immune, isotype control)

20011-25 25 mg
EUR 469

Goat IgG, purified (serum non-immune, isotype control)

20011-5 5 mg
EUR 286

Control Monkey Serum IgA (Rhesus, non-immune), purified

20014-1 25 ug
EUR 225

Turkey IgG, purified (non-immune, isotype control) Serum

20115-1 1 mg
EUR 141

Turkey IgM, purified (non-immune, isotype control) Serum

20115-2-1 25 ug
EUR 225

Turkey IgG, purified (non-immune, isotype control) Serum

20115-5 5 mg
EUR 225

Bactrian camel (Camelus bactrianus; normal, non-immune) serum

NBCS-1 1 ml
EUR 286

Mouse serum (non-immune/control), C57BL6 (heat-inactivated)

NMOS-1CS-H 1 ml
EUR 164

Rabbit Serum

1267-1
EUR 157

Rabbit Serum

abx098265-100ml 100 ml
EUR 272

Rabbit Serum

abx098266-500ml 500 ml
EUR 481

Non-Immune Rabbit IgG Secondary Antibody

A16001 0.5 ml Ask for price
Description: Ask the seller for details

Bovine IgG1-biotin conjugate (Serum Control, non-immune) purified

20001-G1-B 100 ug
EUR 225

Bovine IgG1-HRP conjugate (Serum Control, non-immune) purified

20001-G1-HRP 100 ug
EUR 225

Bovine IgG2-biotin conjugate (Serum Control, non-immune) purified

20001-G2-B 100 ug
EUR 225

Bovine IgG2-HRP conjugate (Serum Control, non-immune) purified

20001-G2-HRP 100 ug
EUR 225

G. Pig IgG, purified (non-immune, serum, Isotype control)

20004-1 1 mg
EUR 141

G. Pig IgM, purified (non-immune, serum, Isotype control)

20004-2-1 0.5 mg
EUR 225

G. Pig IgA, purified (non-immune, serum, Isotype control)

20004-5-1 100 ug
EUR 347

Mouse serum (non-immune/control), C57BL6 (heat-inactivated & delipidized)

NMOS-1CS-HD 1 ml
EUR 202

Normal Rabbit Serum

88-NR50 100 ml
EUR 150
Description: Normal Rabbit Serum

Normal Rabbit Serum

88R-1004 10 ml
EUR 206
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum

89R-RM001 20 mg
EUR 392
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RM002 5 ml
EUR 241
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum

89R-RR001 20 mg
EUR 349
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum

89R-RR002 5 ml
EUR 241
Description: Normal Rabbit Serum (Rat cell adsorbed)

Rabbit Serum Albumin

30R-3303 10 mg
EUR 187
Description: Purified native Rabbit Serum Albumin

Rabbit Complement Serum

32R-CR001 25 ml
EUR 241
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Rabbit Serum (Sterile)

20-abx800002
  • EUR 1400.00
  • EUR 3293.00
  • EUR 829.00
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Sterile)

abx800002-1L 1 L
EUR 5437

Ferret Serum IgM (Control, non-immune), semi-pure for ELISA

20022-1 1000 units
EUR 286

Ferret Serum IgA (Control, non-immune), semi-pure for ELISA

20023-1 1000 units
EUR 347

Rabbit Circulating Immune Complexes ELISA kit

E04C0241-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Circulating Immune Complexes in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Circulating Immune Complexes ELISA kit

E04C0241-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Circulating Immune Complexes in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Circulating Immune Complexes ELISA kit

E04C0241-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Circulating Immune Complexes in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti Bovine Serum antibody

40R-RB001 2 ml
EUR 241
Description: Rabbit anti Bovine Serum antibody

Rabbit Serum Albumin Polyclonal Antibody

A57592 100 µg
EUR 570.55
Description: fast delivery possible

Monkey Serum IgM-biotin conjugate (Rhesus, non-immune, isotype control), purified

20013-1-B 25 ug
EUR 225

Monkey Serum IgM-biotin conjugate (Cyno., non-immune, isotype control), purified

20018-1-B 100 ug
EUR 263

Rabbit anti Pig Serum

40R-RS001 2 ml
EUR 241
Description: Rabbit anti Pig Serum antibody

Rabbit Serum (Non-Sterile

20-abx800001
  • EUR 1080.00
  • EUR 2472.00
  • EUR 662.00
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Non-Sterile

abx800001-1L 1 L
EUR 4281

Non-sterile rabbit serum

RS05-0100 100 ml
EUR 166.4

Non-sterile rabbit serum

RS05-0500 500ml
EUR 192.4

Pre-IL18 (Pre-IL18) Antibody

abx236764-100ug 100 ug
EUR 551

Pre-IL18 (Pre-IL18) Antibody

abx236765-100ug 100 ug
EUR 551

Rabbit IgM (isotype control, non-immune), purified

20009-2-1 0.1 mg
EUR 164

Rabbit IgA (isotype control, non-immune), purified

20009-3-1 0.1 mg
EUR 286

Rabbit IgG (Whole, non-immune)-Biotin conjugate

20009-B 100 ug
EUR 164

Rabbit serum albumin antibody ELISA kit

E04S0304-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit serum albumin antibody ELISA kit

E04S0304-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit serum albumin antibody ELISA kit

E04S0304-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

anti-Rpn3 (yeast) antibody, rabbit serum

62-201 100uL
EUR 453
Description: The anti-Rpn3 (yeast) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

Anti-Streptolysin O antibody rabbit serum

64-001 50uL
EUR 414
Description: The Anti-Streptolysin O antibody rabbit serum is available in Europe and for worldwide shipping via Gentaur.

anti-Rad18 (human) antibody, rabbit serum

70-023 50uL
EUR 414
Description: The anti-Rad18 (human) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

anti-RanBPM (human) antibody, rabbit serum

71-001 50uL
EUR 401
Description: The anti-RanBPM (human) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

anti-RanBP2 (human) antibody, rabbit serum

71-003 50uL
EUR 401
Description: The anti-RanBP2 (human) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

Rabbit Pre Albumin ELISA kit

E04P0054-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Pre Albumin ELISA kit

E04P0054-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Pre Albumin ELISA kit

E04P0054-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Pre Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Pre-Hpt ELISA Kit

ERTP0723 96Tests
EUR 521

Human Immune ribonucleic acid/immune RNA ELISA kit

E01I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Immune ribonucleic acid/immune RNA ELISA kit

E01I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Immune ribonucleic acid/immune RNA ELISA kit

E01I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Immune ribonucleic acid/immune RNA ELISA kit

E03I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Immune ribonucleic acid/immune RNA ELISA kit

E03I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Immune ribonucleic acid/immune RNA ELISA kit

E03I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Immune ribonucleic acid/immune RNA ELISA kit

E02I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Immune ribonucleic acid/immune RNA ELISA kit

E02I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Immune ribonucleic acid/immune RNA ELISA kit

E02I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Immune ribonucleic acid/immune RNA ELISA kit

E08I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Immune ribonucleic acid/immune RNA ELISA kit

E08I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Immune ribonucleic acid/immune RNA ELISA kit

E08I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Immune ribonucleic acid/immune RNA ELISA kit

E06I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Immune ribonucleic acid/immune RNA ELISA kit

E06I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Immune ribonucleic acid/immune RNA ELISA kit

E06I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Immune ribonucleic acid/immune RNA ELISA kit

E07I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Immune ribonucleic acid/immune RNA ELISA kit

E07I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Immune ribonucleic acid/immune RNA ELISA kit

E07I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Immune ribonucleic acid/immune RNA ELISA kit

E09I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Immune ribonucleic acid/immune RNA ELISA kit

E09I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Immune ribonucleic acid/immune RNA ELISA kit

E09I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Low density lipoprotein immune complex ELISA kit

E04L0216-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Low density lipoprotein immune complex in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Low density lipoprotein immune complex ELISA kit

E04L0216-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Low density lipoprotein immune complex in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Low density lipoprotein immune complex ELISA kit

E04L0216-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Low density lipoprotein immune complex in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Anti-Rabies Virus antiserum (hyper immune sera)

RBV12-S 100 ul
EUR 457

Rabbit Serum Iron ELISA kit

E04S0273-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit

E04S0273-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit

E04S0273-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit

E04S0014-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit

E04S0014-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit

E04S0014-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit

E04S0228-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit

E04S0228-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit

E04S0228-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Normal Rabbit Serum (Blocking Solution)

AR1010 100mL (for 10000-15000 assays)
EUR 131

Rabbit Serum S TH 100mL

31126-1 100mL
EUR 175.93

Rabbit Serum S TH 500mL

31126-5 500mL
EUR 230.3

Rabbit serum albumin lyophilized powder

RSA62-0001 1gm
EUR 231.4

Rabbit serum albumin lyophilized powder

RSA62-0010 10gm
EUR 839.8

Rabbit serum albumin lyophilized powder

RSA62-0100 100gm Ask for price

Rabbit serum albumin lyophilized powder

RSA62-1000 1KG Ask for price

Rabbit Polyclonal Antibody to Human Topoisomerase I (Rabbit Serum)

TG2012-2 250 ul
EUR 380

Rabbit Serum Albumin Polyclonal Antibody, HRP Conjugated

A57593 100 µg
EUR 570.55
Description: reagents widely cited

Rabbit Serum Albumin Polyclonal Antibody, FITC Conjugated

A57594 100 µg
EUR 570.55
Description: Ask the seller for details

Rabbit Serum Albumin Polyclonal Antibody, Biotin Conjugated

A57595 100 µg
EUR 570.55
Description: The best epigenetics products

anti-Swi6 (fission yeast) antibody, rabbit serum

63-101 50ul
EUR 375
Description: The anti-Swi6 (fission yeast) antibody, rabbit serum is available in Europe and for worldwide shipping via Gentaur.

Anti-NADase (hemolytic streptococcus) antibody rabbit serum

64-005 50uL
EUR 414
Description: The Anti-NADase (hemolytic streptococcus) antibody rabbit serum is available in Europe and for worldwide shipping via Gentaur.

Anti-Bovine Serum Albumin Rabbit Monoclonal Antibody

M01245 100ug/vial
EUR 397
Description: Rabbit Monoclonal Bovine Serum Albumin Antibody. Validated in IHC, WB and tested in Cow, Human, Mouse, Rat.

Anti-Serum Amyloid A Rabbit Monoclonal Antibody

M01362 100ug/vial
EUR 397
Description: Rabbit Monoclonal Serum Amyloid A Antibody. Validated in WB and tested in Human.

Guinea pig Immune ribonucleic acid/immune RNA ELISA kit

E05I0405-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Guinea pig Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Immune ribonucleic acid/immune RNA ELISA kit

E05I0405-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Guinea pig Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Immune ribonucleic acid/immune RNA ELISA kit

E05I0405-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Guinea pig Immune ribonucleic acid/immune RNA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Immune ribonucleic acid/immune RNA,iRNA ELISA Kit

201-12-1629 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Immune ribonucleic acid/immune RNA(iRNA)ELISA Kit

GA-E1645HM-48T 48T
EUR 289

Human Immune ribonucleic acid/immune RNA(iRNA)ELISA Kit

GA-E1645HM-96T 96T
EUR 466

We consider that the scope of vaccine analysis needs to be broadened towards inhalable dry powder formulations since dry vaccines bear a number of benefits. Firstly, their dry state can tremendously improve vaccine stability and shelf-life. Secondly, they are often inhaled utilizing disposable inhalers, omitting the necessity for skilled well being-care personnel and, due to this fact, facilitating mass-vaccination campaigns. Thirdly, inhalable vaccines might present improved safety since they’ll induce an IgA-mediated mucosal immune response.

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